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KMID : 1134820140430091388
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Journal of the Korean Society of Food Science and Nutrition
2014 Volume.43 No. 9 p.1388 ~ p.1393
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Enzymatic Production of Amylopectin Cluster Using Cyclodextrin Glucanotransferase
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Lee Hye-Won
Jeon Hye-Yeon
Choi Hye-Jeong
Shim Jae-Hoon
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Abstract
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To enzymatically prepare amylopectin cluster (APC), cyclodextrin glucanotransferase (CGTase I-5) and its mutant enzyme from alkalophilic Bacillus sp. I-5 were employed, after which the hydrolysis patterns of CGTase wild-type and its mutant enzyme toward amylopectin were investigated using multi-angle laser light scattering. CGTase wild-type dramatically reduced the molecular weight of waxy rice starch at the initial reaction, whereas the mutant enzyme degraded waxy rice starch relatively slowly. Based on the results, the molecular weight of one cluster of amylopectin could be about 104～105 g/mol. To determine production of cyclic glucans from amylopectin, matrix-assisted laser desorption ionization time-of-flight mass spectrometry was performed. CGTase I-5 produced various types of cyclic maltooligosaccharides from amylopectin, whereas the mutant enzyme hardly produced any.
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KEYWORD
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cyclodextrin glucanotransferase, amylopectin cluster, amylopectin, starch, hydrolysis
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